Map Of The Human Methylome

Welcome to the Map of the human methylome. Methyl Binding Domain based purification of methylated DNA-fragments, followed by second-generation sequencing (MethylCap, also called MiGS and MBDSeq), is one of the most cost-efficient and sensitive methods for completely genome-wide DNA-methylation profiling. This methodology is therefore rapidly gaining interest in the Epigenetics community. Over the course of the last two years, our lab has optimized this technique and the corresponding data analysis. A special peak-calling algorithm was created which involved the indirect use of the underlying CpG-density, and therefore yields far more relevant information than the application of e.g. ChIP-seq peak calling algorithms. On this website, you’ll find current and former releases, in addition. A Genome Browser is available to study our human methylome by browsing to genes and genomic regions.

MHM Methylome Build 2 – 80 samples with MethylCap-Seq kit – 03/02/2012

MHM version 2

This version of the human methylome comprises 80 samples with the following properties:

Enrichment Kit

All samples were enriched with the MethylCap-Seq kit from Diagenode

Samples Origin

Disease Tissue origin Type Number of samples
Cancer colon cell line 1
Colorectal Cancer colon primary sample 7
Neuroblastoma brain primary sample 8
Prostate cancer prostate cell line 2
Cancer blood plasma primary sample 2
Cancer breast cell line 8
Breast cancer breast primary sample 10
Basal cell carcinoma skin primary sample 5
Normal skin Primary sample 4
Normal nose polyps primary sample 6
Normal blood plasma primary sample 2
Normal kidney primary sample 1
Normal melanocytes primary sample 1
Normal stem cell primary cell culture 6
Normal monocytes primary sample 8
Normal thyroid cell line 9

 Downloads

Variables in Bed Format: MHM_VARIABLES_V2.bed

The raw sum of all the peaks is available here: MHM_raw_peaks.tar.gz

MHM Methylome Build 1 – 69 samples with MethylCollector kit – 01/10/2011

MHM version 1

This version of the human methylome comprises 69 samples with the following properties:

Enrichment Kit

All samples were enriched with the Methylcollector Kit from Active Motif

Samples Origin

Disease Tissue origin Type Number of samples
cancer colon cell line 18
cancer colon primary sample 6
cancer prostate cell line 4
cancer kidney cell line 7
cancer breast cell line 8
cancer skin cell line 5
normal colon primary sample 2
normal nose polyps primary sample 6
normal blood serum primary sample 2
normal breast cell line 1
normal breast primary sample 1
normal stem cell primary cell culture 8
normal skin cell line 1

Downloads

Variables in Bed Format: MHM_VARIABLES_V1.bed